DNA of semen and quality of the oocyte

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The effects of semen DNA fragmentation depend on the quality of the oocyte.

In this study we have analyzed the effect of sperm DNA fragmentation on reproductive outcomes. For this, 210 seeds have been analyzed whose pairs are subjected to IVF - ICSI cycles, both using fresh or frozen semen and their own or donor oocytes.

With this, it has been observed that the rate of fragmentation of the DNA is not altered with the freezing of the semen in a significant way and that these levels show no difference after performing the swim-up. With the use of own oocytes, it was found that if the levels of fragmentation increased, a decrease in the probability of pregnancy was observed. In the case of donor oocytes, this decrease was minor but not significant.

This shows that the effect of sperm DNA fragmentation does not depend on the origin of the semen or on the fertilization process used and that its effects can be diminished by the reparative capacity of the oocytes.

Below you have an index with all the points that we will discuss in this article.

Index

Material and methods

In this study, a total of 210 couples undergoing IVF-ICSI have been evaluated. The inclusion criteria for the study were: female couple aged between 30 and 45 years, undergoing a first cycle of IVF and with a Body Mass Index less than 30Kg / m2. The embryo transfer of the studied cycles was always carried out at 72 hours after the oocyte recovery. The study excluded women with some type of uterine pathology, endocrine dysfunction, recurrent abortions and severe oligospermias.

To evaluate the level of sperm DNA fragmentation, the Halosperm commercial Kit was used. To the samples of semen, both those used in fresh and frozen, they were carried out the fragmentation test before and after being trained by swim-up.

A total of 182 ICSI cycles and 28 cycles in which IVF was performed were analyzed. Of these, in 112 donor oocytes were used and in 98 oocytes from the patient herself. In all of them, the clinical pregnancy was confirmed by the presence of a gestational sac with a heartbeat at 7 weeks of pregnancy. It was considered a miscarriage when the pregnancy did not reach week 22.

Bibliography

Meseguer M, Santiso R, Garrido N, García-Herrero S, Remohí J, Fernandez JL. Effect of sperm DNA fragmentation on pregnancy outcome depends on oocyte quality. Fertil Steril. 2011; 95 (1): 124-8

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