How is the amount of sperm in the semen analyzed?

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To determine how many sperm there are in an ejaculation it is necessary to make a semengram. This diagnostic test of male fertility allows to assess if there is a sufficient concentration of sperm to achieve a natural pregnancy, in addition to other seminal parameters.

The amount of sperm in the semen of the male is important at the time of getting a pregnancy since many die during the way to the egg.

The vagina, the passage through the cervix and the arrival to the fallopian tubes are a biological gynaecine in which many sperm do not reach the destination, so it is important to start the race with a good sperm concentration.

Below you have an index with all the points that we will discuss in this article.

Index

How is the amount of sperm in an ejaculate analyzed?

The spermatic count

Once the semen sample has been obtained in a sterile bottle, it remains at rest in the laboratory for about 20 minutes to favor fluid liquefaction. The temperature must be adequate so that the sperm do not lose their mobility and can give reliable results.

Next, a drop of semen is taken and placed on a counting chamber to observe it under a microscope.

The most used camera for counting sperm is the Makler camera, which consists of a perfectly defined grid of 10 rows x 10 columns that facilitates counting.

The counting method consists of counting the sperm there are for every 10 frames, that is, in each row or column. This result must be multiplied by the factor 106 / ml (1 million per milliliter) and, finally, the sperm concentration expressed in millions of spermatozoa per ml is obtained.

To make the count more reliable, it is necessary to count several rows or columns of the grid and make a mean with all the results obtained. In the same way, there are specialists who prefer to count on two different semen drops so that the result is closer to reality.

Counting chamber to evaluate the concentration of sperm

Finally, to know the total amount of sperm that contains an ejaculation, it will be necessary to multiply the sperm concentration obtained by the volume of the semen sample.

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