Long embryo culture and classification of blastocysts

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In recent years, long cultivation in in vitro fertilization (IVF) treatments has gained special relevance because better implantation rates are obtained with blastocyst transfers (day 5-6 of development) than with embryos in earlier stages. .

One of the main advantages is that it allows to improve the embryonic selection. To do this, the blastocysts are classified according to their morphological quality and their degree of expansion, that is, at what stage of development they are: early, cavitated, expanded, hatched, etc.

Below you have an index with all the points that we will discuss in this article.

What is long blastocyst culture?

The long embryo culture consists of keeping the embryos in the laboratory until the 5th or 6th day of their development, when they reach the blastocyst stage.

In the blastocysts we can clearly differentiate two structures, that is, it is possible to distinguish:

Trofoectodermoson the blastocyst cells that come in contact with the endometrium and, later, this structure will originate the placenta. On the outside, it is protected by the zona pellucida. Cellular internase lies inside the trophoectoderm and will lead to the fetus.

When the embryo is implanted naturally in the endometrium, it is in the blastocyst stage. If the embryonic development is carried out in the IVF laboratory, in order for the embryo to reach the blastocyst stage, it must be maintained under optimal culture conditions so that they are of good quality and implanted when transferred to the maternal uterus.

Parts of the blastocyst

Types of cultivation

Long blastocyst culture can be performed with two types of culture media:

Sequential culture

In order to achieve that the embryos reach the blastocyst stage, they are cultivated in two media with different chemical compositions that adapt to the nutritional requirements of the embryos at each moment of their development.

The embryos will be cultured in the first medium from their zygote stage (day 1) until day 3 of development. In the second, from day 3 to the blastocyst stage (day 5-6).

Questions from users


embryo transfer in Barcelona(Spain)

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